Fig. 4: PvAVH53 interacts with Nicotiana benthamiana NbImpαs. | Horticulture Research

Fig. 4: PvAVH53 interacts with Nicotiana benthamiana NbImpαs.

From: Importin-αs are required for the nuclear localization and function of the Plasmopara viticola effector PvAVH53

Fig. 4

a Phylogenetic analyses of the VvImpαs (VvImpα XP_002274422.1, VvImpα2 XP_002282816.1, VvImpα4 XP_002281670.1, VvImpα6-like XP_010646172.1, VvImpα5 XP_002281591.1), and NbImpαs (NbImpα1: EF137253.1 and NbImpα2: EF137254.1) were conducted with MEGA5 software. b Sequence analysis of the VvImpα/α4 and NbImpα1/2. The sequence alignment of the NbImpαs and VvImpαs indicated that VvImpα and VvImpα4 showed high sequence identity with the two NbImpαs. c NbImpα1/2 interact with PvAVH53 in yeast Y2H Gold cells. The yeast cells grew and turned blue on DDO (SD-Leu/-Trp) and QDO/A/X (SD-Ade/-His/-Leu/-Trp) in the presence of Aba and X-α-Gal

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