Fig. 5: NbImpα1/2 silencing reduces plant growth and disturbs the nuclear localization of PvAVH53. | Horticulture Research

Fig. 5: NbImpα1/2 silencing reduces plant growth and disturbs the nuclear localization of PvAVH53.

From: Importin-αs are required for the nuclear localization and function of the Plasmopara viticola effector PvAVH53

Fig. 5

a Morphology of N. benthamiana plants with TRV:GFP (control) and TRV:Impα1/2. b Relative quantification of the expression of NbImpα1/2 with TRV constructs using qRT-PCR. The NbEF1α gene was used as an internal control. (c) Subcellular localization in leaves of control (TRV:GFP) and Impα1/2-silenced plants analyzed by confocal microscopy. Photos were taken after protoplasts were incubated for 20–24 h under weak lighting at 25 °C. Scale bar = 10 μm. The experiments were repeated three times with similar results, and at least two protoplasts were observed each time

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