Fig. 2: CmRCD1 physically interacted with CmBBX8.
From: CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum
a BiFC assay. DIC: images taken in bright light; mRFP-NLS: images taken in the red fluorescence channel; YFP: images taken in the yellow fluorescence channel; merged: both overlay plots; bars = 20 μm. b Pulldown and c CoIP analyses of the interaction between CmRCD1 and CmBBX8. The arrows show the target protein sizes. GST-empty vector & His-CmBBX8 was used as a control for the pulldown assay, and HA-CmBBX8 alone was used as a control for the CoIP assay. The red arrow indicates the size of the target protein
