Fig. 7: Transcription levels of apple autophagy-related genes and autophagosomes accumulation in the leaves of MdATG8i-overexpressing apple lines and WT plants after 80 days of a moderate drought treatment.

a Expression levels of apple autophagy-related genes in the leaves of WT and MdATG8i-overexpressing apple plants under moderate drought conditions. b Transmission electron microscopic (TEM) images of the autophagic structures in the leaves of WT and MdATG8i-overexpressing apple plants. The autophagic structures are shown by black arrows. Scale bars: 1 μm. c Relative autophagic activity represented as a comparison of the activities of the WT or transgenic plants shown in b. The results are represented as the means ± SDs. Values with different letters differed significantly at P < 0.05 according to Tukey’s multiple range test.