Fig. 2: Subcellular localization, transactivation, and expression pattern analysis of CmJAZ1-like.
From: Overexpression of the CmJAZ1-like gene delays flowering in Chrysanthemum morifolium
A Subcellular localization of CmJAZ1-like in onion cells. The coexpressed 35S::D53-RFP construct was used as a nuclear marker. Bar: 50 μm. B Transcriptional activation of CmJAZ1-like in yeast cells. pCL1 was the positive control, and pGBKT7 was the negative control. Left: SD/-Ade-His medium, right: SD/-Ade-His medium coated with X-α-gal. C Expression pattern of CmJAZ1-like in various organs of the wild-type chrysanthemum cultivar ‘Jinba’. CmEF1α (GenBank: AB548817.1) was used as an endogenous control in chrysanthemum. The 2−ΔΔCt method was used to calculate relative transcript abundances. The values are presented as the mean ± SE (n = 3). Significant differences were analyzed using Duncan’s multiple-range test. (P < 0.05)
