Fig. 4: Isoform and alternative splicing (AS) analysis of I. hawkeri leaf transcripts using PacBio Iso-seq. | Horticulture Research

Fig. 4: Isoform and alternative splicing (AS) analysis of I. hawkeri leaf transcripts using PacBio Iso-seq.

From: Integration of early disease-resistance phenotyping, histological characterization, and transcriptome sequencing reveals insights into downy mildew resistance in impatiens

Fig. 4

A Distribution of isoform numbers based on UniTransModels. B Summary of AS events identified in the full-length transcriptome using UniTransModels as the reference. RI retained intron, A5 alternative 5′ splice-site, A3 alternative 3′ splice-site, SE skipping exon, AF alternative first exon, AL alternative last exon. C Sashimi plots showing examples of the six types of AS events. Red peaks indicate overage of Illumina short reads; curved red lines with numbers represent splicing junctions supported by this number of Illumina short reads. For isoforms located at the bottom, blue blocks represent exons and lines linking blocks represent introns

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