Fig. 6: Gene expression profiles for impatiens cotyledons and true leaves, and differentially expressed genes between downy mildew-susceptible vs. -resistant samples.

A Number of expressed transcripts (transcripts per million or TPM > 0.5) for each tissue type and each cultivar. DOB Divine Orange Bronze Leaf, SER Super Elfin Red, FLR Florific Lavender. Green circle represents cotyledon; green triangle represents true leaf. “+” indicates expressed; “−” indicates not expressed. The “No. of transcripts” (from top to bottom) corresponds to the transcripts that were only expressed in true leaves and not expressed in cotyledons for all three genotypes; transcripts that were only expressed in true leaves of Impatiens hawkeri and not expressed in cotyledons of Impatiens hawkeri, neither expressed in cotyledons nor true leaves of Impatiens walleriana; transcripts that were only expressed in cotyledons and not expressed in true leaves for all three genotypes; transcripts that were only expressed in cotyledons of Impatiens hawkeri and not expressed in true leaves of Impatiens hawkeri, neither expressed in cotyledons nor true leaves of Impatiens walleriana. B Principal component analysis of all genes using DESeq2 with normalized counts. Circles with different colors represent different groups of samples as shown in the figure. C Upregulated genes based on five downy mildew-susceptible vs. resistant sample comparisons. D Downregulated genes based on five susceptible and resistant sample comparisons