Fig. 3: Rapid PCR site-directed mutagenesis of FaARF4. | Horticulture Research

Fig. 3: Rapid PCR site-directed mutagenesis of FaARF4.

From: Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry

Fig. 3

a RLM-5ʹ RACE assay showing that tasiRNA3 cleaves FaARF4. The red arrows represent the cleavage sites. The numbers under the red arrows represent the number of clones that possessed the cleavage site and the total number of clones in the RLM-5ʹ RACE assay. b Nucleotide and amino acid sequence analysis of FaARF4mut and FaARF4. The red colons indicate the nucleotides that differ between FaARF4mut and FaARF4. The dotted lines represent complementary sequences

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