Fig. 2: Schematic workflow of the DiRT pipeline to identify dicistronic candidates from RNA-seq data. | Horticulture Research

Fig. 2: Schematic workflow of the DiRT pipeline to identify dicistronic candidates from RNA-seq data.

From: Tissue and regional expression patterns of dicistronic tRNA–mRNA transcripts in grapevine (Vitis vinifera) and their evolutionary co-appearance with vasculature in land plants

Fig. 2

tRNA gene and protein-coding gene coordinates were retrieved from GtRNAdb and Ensembl Plants, respectively. tRNA-protein-coding gene pairs that occupied contiguous spaces in the genome were assembled and selected for subsequent analysis if they were transcribed based on RNA-seq reads (Raw read ≥ 1 for tRNAs and raw read ≥ 10 in PCGs). Dicistronic tRNA–mRNA are identified by assessing active transcription of the intergenic region. Candidate tRNA–mRNA are selected if the intergenic regions had significantly higher expression (FDR < 0.05) than the closest two introns. tRNA and protein-coding gene Pairs are classified as putatively dicistronic tRNA–mRNA if the intergenic region showed continuous sequencing coverage between the tRNA and the mRNA of the protein-coding gene

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