Fig. 2: Hyperoside prolonged the flowering period and increased the effective pollination rate and pollen tube growth.

A Schematic chart of the prolonged effective pollination period after adding hyperoside. B Time-lapse images of a flower from an untreated plant, a flower from a plant treated with buffer solution only, and a flower from a plant treated with a buffer solution containing 50 mg/l hyperoside. The processed photos of each group were taken on the same day. The scale bars are 1.5 cm. C The effective pollination period was analyzed in the control, buffer addition, and hyperoside addition groups. D, E The average interior diameter and opening angle of the flowers were analyzed at 9:00, 10:00. and 16:00. The changes in total flavonoids were also measured in the three groups. F The average length of pollen tubes in the style of flowers from control plants and plants after spraying buffer or hyperoside for 40 min. In C–F, each treatment had three biological replicates, and error bars display the standard error of the sample. *P < 0.05, **P < 0.01 (Student’s t test)