Fig. 1: Identification of candidate Populus trichocarpa SVL homologs and overexpression construct design. | Horticulture Research

Fig. 1: Identification of candidate Populus trichocarpa SVL homologs and overexpression construct design.

From: Overexpression of SHORT VEGETATIVE PHASE-LIKE (SVL) in Populus delays onset and reduces abundance of flowering in field-grown trees

Fig. 1

A Phylogeny of eudicot proteins in the StMADS11 superclade26. Representative eudicot members of the StMADS11 superclade were obtained by BLAST P searches of the following Phytozome v13 (https://phytozome-next.jgi.doe.gov/) proteomes (protein ID prefix): Populus trichocarpa v4.1 (Potri, shown in orange), Aquilegia coerulea v3.1 (Aqcoe), Solanum lycopersicum ITAG3.2 (Solyc), Solanum tuberosum v4.03 (PGSC), Mimulus guttatus NONTOL v4.0 (MgNOTOL), Eucalyptus grandis v2.0 (Eucgr), Vitis vinifera v2.1 (VIT), Prunus persica v2.1 (Prupe), Arabidopsis thaliana Araport11 (AT). Bootstrap values equal or greater than 50% are shown at nodes. Full length protein sequences were aligned with MUSCLE45. A maximum likelihood phylogenetic analysis was performed on the sequence alignment using the JTT + G model, 75% deletion of alignment gaps/missing data and 100 bootstraps for branch support testing with the program MEGA X46. Arrow indicates P. trichocarpa protein chosen for overexpression in this study. B Architecture of the SVL overexpression construct. LB T-DNA left border, p35S Cauliflower mosaic virus 35S promoter, nosT nopaline synthase terminator, ocsT octopine synthase terminator, RB T-DNA right border

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