Fig. 2: Representative images of field grown SVL overexpressing transgenic poplars.

A Examples of field grown poplars illustrating the floral scoring system used to determine flowering presence and abundance prior to bud break in spring. Trees were scored from 0 to 1, then converted for quantitative analysis as 0 to 0.835 (see “Methods” section) based on the increasing coverage of catkins within the crowns of established trees. B–D Representative examples of SVL overexpressors with delayed flowering adjacent to flowering individuals of the same age produced with constructs that did not appear to affect onset or abundance of flowering. Flowering neighboring transgenic trees from unrelated constructs in B and C are indicated by purple arrows. Non-flowering 35S:SVL transgenics are indicated by an orange arrow. Images were taken in the spring of 2017, 6 years after field planting, with a Canon Rebel XSI digital camera