Fig. 6: Identification of hormone-responsive transcription factors regulating melatonin synthase genes by dual-luciferase assay. | Horticulture Research

Fig. 6: Identification of hormone-responsive transcription factors regulating melatonin synthase genes by dual-luciferase assay.

From: Metabolite profiling and transcriptome analyses reveal novel regulatory mechanisms of melatonin biosynthesis in hickory

Fig. 6

A Diagrams of the reporter and effector vectors used in dual-luciferase assays. B CcEIN3-1 and CcEIN3-2 activate the promoter of CcTDC1. C CcAZF2 activates the promoter of CcASMT1. The LUC/REN value of the empty vector on each promoter was set as 1 as a calibrator. Each value represents the mean ± SD of three independent experiments, with three replicates in each experiment. Statistical significance was determined by Student’s two-tailed t-test (*P < 0.05, **P < 0.01)

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