Fig. 3: Sclareol and linalyl acetate ¹³C-labeling after feeding with 1-¹³C-glucose and terpene biosynthesis inhibitors.

Clary sage cut inflorescence stems were fed with a solution containing 1-¹³C-glucose or standard glucose with 100 µM of mevinolin, 100 µM of fosmidomycin, or no inhibitor (control) for 5 days. Sclareol (a) and linalyl acetate (b) ¹³C-labeling was analyzed by quantitative ¹³C nuclear magnetic resonance (¹³C-NMR) after hexane extraction. The labeling ratio was calculated as follows: (Peak area_{labeled glucose} − Peak area_{standard glucose})/Peak area_{standard glucose}. Bars represent the mean ± SD (n = 3)