Fig. 4: Localization of sclareol and linalyl acetate at the surface of a clary sage calyx.

The chemical composition of the epidermal surface of clary sage calyces was analyzed through laser desorption–ionization followed by FT-ICR mass spectrometry (LDI–FT-ICR). a Representative mass spectrum of the surface of a clary sage calyx. Spatial distribution of [L+K]⁺ ions (b), [L+L+K]⁺ ions (d), [S+K]⁺ ions (c) [S+S+K]⁺ ions (e) and at the surface of the calyx sample. f Overlay of [L+K]⁺ and [L+L+K]⁺ ions. g Overlay of [S+K]⁺ and [S+S+K]⁺ ions. h Top view of a clary sage calyx sample observed with a zoom stereomicroscope. i Overlay of [L+K]⁺, [L+L+K]⁺, [S+K]⁺, and [S+S+K]⁺ ions. Blue arrowheads indicate short capitate glandular trichomes, circles indicate large capitate glandular trichomes, dashed circles indicate peltate glandular trichomes. L linalyl acetate, S sclareol, Scale bar: 100 µm