Fig. 5: MEP and sclareol biosynthesis genes are expressed in isolated glandular trichome heads.
From: Sclareol and linalyl acetate are produced by glandular trichomes through the MEP pathway
a, b Clary sage calyx before (a) and after (b) abrasion of the surface with glass beads. c Purified glandular trichome heads. Scale bar: 200 µm. d, e Relative gene expression of MEP (d), sclareol (e), and photosynthesis (f) genes in calyx before abrasion (dark green) and purified glandular trichome heads (orange). MEP biosynthesis genes: DXS2 1-deoxy-d-xylulose-5-phosphate synthase 2, DXR 1-deoxy-d-xylulose-5-phosphate reductoisomerase, MCT 2-methyl-d-erythritol-4-phosphate cytidylyltransferase, CMK 4-diphosphocytidyl-2-methyl-d-erythritol kinase, HDS 4-hydroxy-3-methylbut-2-enyl-diphosphate synthase, HDR 4-hydroxy-3-methylbut-2-enyl-diphosphate reductase. Sclareol biosynthesis genes: GGPPS geranylgeranyl-diphosphate synthase, LPPS labda-13-en-8-ol-diphosphate synthase, ScS sclareol synthase. Photosynthetic genes. RbcS ribulose-bisphosphate carboxylase small chain, CAB chlorophyll a-b binding protein. Bars represent the mean ± SD (n = 4). Stars indicate the results of Student’s t test (*p < 0.05, **p < 0.01)
