Fig. 5: Effects of ClCOMT1 gene-editing on endogenous melatonin contents in watermelon calli. | Horticulture Research

Fig. 5: Effects of ClCOMT1 gene-editing on endogenous melatonin contents in watermelon calli.

From: The role of watermelon caffeic acid O-methyltransferase (ClCOMT1) in melatonin biosynthesis and abiotic stress tolerance

Fig. 5

A Two sgRNAs (Target 1 and Target 2) in the 5’-region of ClCOMT1. B Schematic diagram for constructing two sgRNA cassettes in the binary vector PBSE402. C Mutation types and rates in the transgenic watermelon calli after CRISPR/Cas9-mediated gene editing. D Melatonin contents in transgenic and wild-type (WT) calli. In (D), values are means ± SD (n = 3). Asterisk (*) indicates significant difference at P < 0.05. ClCOMT Citrullus lanatus caffeic acid O-methyltransferase

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