Fig. 2: MdPR10-1 and MdPR10-2 physically interact with MdRNL2–MdRNL6 at the LRR domain.

A Y2H analysis showing the interactions between MdPR10-1 and MdRNL2-3, MdPR10-2 and MdRNL2-3, MdPR10-1 and MdRNL6-3, and MdPR10-2 and MdRNL6-3. B β-Galactosidase activity in Y2H assays. The error bars show the standard error of the mean (SEM) values (n = 3 biological replicates). **P < 0.01 (Student’s t test). C BiFC assay of the interactions between MdPR10-1 and MdRNL2-3, MdPR10-2 and MdRNL2-3, MdPR10-1 and MdRNL6-3, and MdPR10-2 and MdRNL6-3 when transiently expressed in Nicotiana benthamiana epidermal cells. Scale bars = 50 μm