Fig. 4: Recombinant MdPR10-1 and MdPR10-2 proteins have anti-ALT1 activity in vitro.

A Growth inhibition of ALT1 treated with MdPR10-1 and MdPR10-2 proteins. Left: 20 μL of ALT1 sporangial suspension + 60 μL of 500 μg/μL purified MdPR10-1/MdPR10-2 proteins. Right: 20 μL of ALT1 sporangial suspension + 60 μL of 500 μg/μL of purified GST proteins. B Area of ALT1 mycelium growth in PDA, as determined using ImageJ. The spore suspension concentration was 2 × 105 CFU/mL. The data were collected on day 6 in PDA. Scale bars = 1 cm. Student’s t test: **P < 0.01