Fig. 3: E15 determined the catalytic activity of GbNGT1.
From: N-glucosyltransferase GbNGT1 from ginkgo complements the auxin metabolic pathway
a The binding domain of GbNGT1 docking with UDPG and IAA-Asp; the molecule in yellow and orange is UDPG; the molecule in pink is IAA-Asp. b HPLC chromatograms of enzymatic products, which included native or mutant, UDPG, and IAA-Asp. c HPLC chromatograms of enzymatic products, which included native or mutant, UDPG, and IAA. d The kinetic data of native and mutants toward IAA-Asp. The molecular weights of recombinant GbNGT1 and mutants with MBP tags were all ~97.72 kDa (MBP tag molecular weight was 42.5 kDa, average was used (SD), n = 3. Red arrow, product; green arrow, substrate
