Fig. 3: Identification and characterization of FveSEP3.

a Diagram showing the locations of high-quality exonic SNPs. The red line indicates the candidate causative SNP in FvH4_4g23530 (FveSEP3) that results in the conversion of G to A in DNA and G (Gly) to E (Glu) in protein. The arrows indicate the positions of primers used for examining the mutation. b Phylogenetic tree of the SEP proteins of F. vesca, Arabidopsis, tomato, and apple using full-length sequences. c Sequence logo of the first 40 amino acids in the MADS domain based on 18 homologs showing the conserved amino acids. The relative heights of letters indicate the frequencies that appeared in those sequences. d Subcellular localization of FveSEP3-GFP in tobacco leaves. Green indicates GFP fluorescence, and red indicates mCherry fluorescence from the nuclear marker VirD2NLS-mCherry. Scale bars: 50 μm. e Expression levels (TPMs) of the four FveSEP genes in F. vesca according to the RNA-seq analysis