Fig. 5: Interactions between FveSEP3 and the ABCE class of MADS-box proteins. | Horticulture Research

Fig. 5: Interactions between FveSEP3 and the ABCE class of MADS-box proteins.

From: The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry

Fig. 5

a Relative expression levels of the class ABCE genes in stage 9–10 flowers of wild-type and R27/fvesep3 strawberry plants were determined using qRT-PCR analysis. Data are the means ± SD obtained from three biological replicates. **, P < 0.01, Student’s t test. b, c Physical interactions between FveSEP3 and class ABCE MADS-box proteins in strawberry were examined by yeast two-hybrid assay. Transformed yeast cells were grown on SD-Leu-Trp (DDO) and SD-Leu-Trp-His-Ade (QDO). AD, activation domain, BD, DNA-binding domain. Empty vectors were used as controls. d Physical interactions between FveSEP3 and class ABCE MADS-box proteins (labeled below) were examined by split luciferase assay. The genes were transiently expressed in tobacco leaves. On the left are the negative controls. On the right are the interaction signals between nLUC-SEP3 and A/B/C/E-cLUC

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