Fig. 7: Transcriptome analysis of fruit at 6–7 DPA.

a Fruit samples used for RNA-seq. b Venn diagram showing the differentially expressed genes in fvesep3 and pollinated wild-type fruit compared to unpollinated wild-type fruit. c Top 15 enriched GO terms (corrected P value < 0.01) among the upregulated genes in fvesep3 and pollinated wild-type fruit compared to unpollinated wild-type fruit. d Heatmap showing the expression levels of the differentially expressed auxin and GA pathway genes. The Z-score obtained from TPM values was used to present the expression levels. e Validation of the expression levels of the differentially expressed genes for auxin biosynthesis, transport, and signaling by qRT-PCR. Data are the means ± SD obtained from three biological replicates. **, P < 0.01, Student’s t test