Fig. 5: Transient expression assays verifying the function of PuRBOHF in Nanguo pear fruit and flesh calli. | Horticulture Research

Fig. 5: Transient expression assays verifying the function of PuRBOHF in Nanguo pear fruit and flesh calli.

From: Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit

Fig. 5

a–d PuRBOHF overexpression or antisense constructs were injected into the fruit flesh at 40 days after full bloom using needleless syringes. Infiltrated sites: A, B, and C; corresponding noninfiltrated sites: a, b, and c. a Phloroglucinol-HCl staining of lignin and b DAB staining of reactive oxygen species, observed 10 days after infiltration. Expression of (c) PuRBOHF and (d) lignin and H2O2 contents in the flesh around the infiltration sites and corresponding noninfiltrated sites. e–g Overexpression of PuRBOHF in pear calli. e Relative PuRBOHF transcript levels and f lignin contents in PuRBOHF-overexpressing calli. g Relative expression of lignin biosynthesis-related genes in transgenic PuRBOHF-overexpressing pear calli. Error bars: standard deviations (three replications). *P < 0.05

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