Fig. 6: Relationship between PuRBOHF-mediated ROS production and the stone cell transcriptional network. | Horticulture Research

Fig. 6: Relationship between PuRBOHF-mediated ROS production and the stone cell transcriptional network.

From: Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit

Fig. 6

a–c Effects of H2O2 on proPuPOD2-GUS and proPuLAC2-GUS promoter activity. a Schematics of GUS reporter gene constructs driven by the PuPOD2 promoter or PuLAC2 promoter. The constructs were transformed into 5-week-old tobacco leaves. The infiltrated tobacco leaves were then sprayed with H2O2 solution (+H2O2) or distilled water (−H2O2). After 3 days of growth, the leaves were harvested for b histochemical GUS staining and c GUS activity analysis. d–f PuMYB169 activated PuRBOHF transcription, as revealed by a dual-luciferase (LUC) assay. d Schematics of the reporter and effector gene constructs used in the dual LUC assay. e In tobacco leaves, transient expression of PuMYB169 activated PuRBOHF promoter activity. The transient expression assay was performed with three replicates. f Quantitative analysis of the luminescence intensity for each image in (e). Error bars: standard deviations (three replications). *P < 0.05

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