Fig. 3: Transient dual-luciferase reporter assay.
From: Transcriptional regulation of flavonoid biosynthesis in Artemisia annua by AaYABBY5
a Schematic representation of constructs used to prepare effector and reporter strains. The AaYABBY5 open-reading frame fused to the yellow fluorescent protein (YFP-N) in pEG104 was used as the effector. YFP-N was used as a negative control. Promoters of PAL, CHS, CHI, DFR, FLS, FSII, LDOX, and UFGT were fused with the LUC gene at its N-terminus as reporter constructs. From b–i showing relative LUC activities obtained for each combination of the reporter with effector. Significant increases were found with the PAL, CHI, CHS, and UFGT promoters. Data show the mean values ± SD of four independent infiltrations. Error bars show the standard deviation for n = 4. **P < 0.01. *P < 0.05. The gene sequence of AaYABBY5 can be found in NCBI GenBank under accession number MK675289. The promoter sequences of DFR, PAL, CHS, CHI, FLS, FSII, LDOX, and UFGT have been submitted to the National Center for Biotechnology Information (NCBI), and accession numbers have been assigned as MW558943, MW558944, MW915581, MW558945, MW464242, MW464241, MW464239, and MW464240, respectively
