Fig. 3: SlZHD17 regulates chlorophyll accumulation and chloroplast development in mature green fruit.
From: SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit
a Photograph of WT and SlZHD17-RNAi (line #11) fruit at different stages. Dpa days post anthesis, MG mature green stage, Br breaker stage, and Br+2, Br+5, Br+7, and Br+10 indicate 2 days, 5 days, 7 days, and 10 days post breaker stage, respectively. b Photograph of WT and SlZHD17-RNAi fruit at the mature green stage. c The contents of chlorophyll a, chlorophyll b, and total chlorophyll in WT and SlZHD17-RNAi fruit at the mature green stage. FW fresh weight. Data represent the mean values of three independent experiments, and error bars show the standard error values. Single asterisk (*) and double asterisks (**) refer to significant differences between WT and transgenic lines with P < 0.05 and P < 0.01, respectively (two-tailed Student’s t-test). d, e Chlorophyll autofluorescence in the exocarp (d) and mesocarp (e) of WT and SlZHD17-RNAi (line #11) mature green fruit observed by confocal laser scanning microscopy. f Chloroplast ultrastructure in the mesocarp of WT and SlZHD17-RNAi (line #11) mature green fruit observed by transmission electron microscopy (TEM). C chloroplasts, P plastoglobules, and T thylakoid grana stacks. g, h Chloroplast length (g) and chloroplast area (h) of WT and SlZHD17-RNAi (line #11) mature green fruit. Data represent the mean values (WT, n = 5; SlZHD17-RNAi, n = 9), and error bars show the standard error values. Single asterisk (*) and double asterisks (**) refer to significant differences between WT and transgenic lines with P < 0.05 and P < 0.01, respectively (two-tailed Student’s t-test)
