Fig. 1: Multiple alignments of MdWRKY75d and MdWRKY75e and phylogenetic analysis of WRKY75s from different plants.
From: MdWRKY75e enhances resistance to Alternaria alternata in Malus domestica
a Amino acid sequence alignment of MdWRKY75d, MdWRKY75e, and WRKY75s from various plant species. The same amino acids are displayed on a black background, and similar amino acids are shown in gray. The WRKY motif is indicated by a line, and the zinc finger structure is marked by a red circle. The sequences used for comparison with MdWRKY75d and MdWRKY75e were Arabidopsis thaliana AtWRKY75 (NP_196812.1), Nicotiana tabacum NtWRKY75 (XP_016446514.1), Populus trichocarpa PtWRKY75 (XP_006373216.1), and Populus euphratica PeWRKY75 (XP_011009191.1). b Construction of a phylogenetic tree on the basis of WRKYs from different plants, including MdWRKY75d and MdWRKY75e. A phylogenetic tree was created with MdWRKY75d, MdWRKY75e, and the following WRKY75 family proteins: Vitis vinifera VvWRKY75 (XP_002275576.1), Populus trichocarpa PtWRKY75 (XP_006373216.1), Populus euphratica PeWRKY75 (XP_011009191.1), Nicotiana tabacum NtWRKY75 (XP_016446514.1), Arabidopsis thaliana AtWRKY75 (NP_196812.1), Raphanus sativus RsWRKY75 (XP_018475395.1), Brassica oleracea var. oleracea BoWRKY75 (XP_013626795.1), Brassica rapa BrWRKY75 (XP_009131315.1), Rosa chinensis RcWRKY75 (XP_024197588.1), Fragaria vesca subsp. vesca FvWRKY75 (XP_004310100.1), Pyrus × bretschneideri PbWRKY75 (XP_009372253.1), Fragaria × ananassa FaWRKY1 (ACH88751.1), Ipomoea nil InWRKY75 (XP_019199847.1), Theobroma cacao TcWRKY75 (XP_007031915.1), Malus baccata MbWRKY75 (AHJ78583.1), Juglans regia JrWRKY75 (XP_018836802.1), Prunus avium PaWRKY75 (XP_021828982.1), Gossypium hirsutum GhWRKY75 (XP_016725404.1), Pyrus ussuriensis × Pyrus communis PuWRKY75 (KAB2599104.1), Gossypium raimondii GrWRKY75 (XP_012446088.1), Prunus mume PmWRKY75 (XP_008222197.1), Prunus persica PpWRKY75 (XP_007226197.1), Prunus dulcis PdWRKY75 (XP_034198908.1), Citrus clementina CcWRKY75 (XP_006437986.1), Solanum tuberosum StWRKY75-like (NP_001275604.1), Solanum pennellii SpWRKY75 (XP_015075730.1), Solanum lycopersicum SlWRKY1 (NP_001310244.1), Glycine max GmWRKY75 (XP_003549123.1), and Zea mays ZmWRKY75 (PWZ27788.1). Bootstrap values are shown for each diverging branch. MdWRKY75d and MdWRKY75d are denoted with asterisks
