Fig. 5: Specific evolution of genes involved in steroid-related derivative biosynthesis in Physalis.

A A simplified steroid-related derivative biosynthetic pathway. The solid arrows indicate the confirmed sections, while the dashed arrows represent the proposed sections. The examined enzymes are given, and the gene numbers in different species are indicated by the histogram. MVA, mevalonic acid; MEP, methylerythritol 4-phosphate; IPI, isopentenyl pyrophosphate isomerase; GPPS, geranyl diphosphate synthase; FPPS, farnesyl diphosphate synthase; SQS, squalene synthase; SQE, squalene epoxidase; CAS, cycloartenol synthase; LAS, lanosterol synthase; HSD, 3-beta hydroxysteroid dehydrogenase/isomerase. B A schematic diagram displaying the origin and expansion of SQE genes in P. floridana. The blue square represents 15 Physalis-specific SQE genes that expanded from a conserved Solanaceae SQE (pf03G072270) but were lost in Solanum. The triangle represents the other four Solanaceae-conserved SQE genes. Detailed information is available in Supplementary Figs. 22 and 23