Fig. 6: MBP21-like MADS-box gene loss and Chinese lantern development in Physalis. | Horticulture Research

Fig. 6: MBP21-like MADS-box gene loss and Chinese lantern development in Physalis.

From: The Physalis floridana genome provides insights into the biochemical and morphological evolution of Physalis fruits

Fig. 6

A The phylogeny of SEP-like MADS-box genes from the indicated plant species. Different subclades are highlighted with different colors. The circles represent the interactions between MPF2 (left)/MPF3 (right) and the corresponding SEP-like proteins. Blue represents the interaction signals detected in yeast two-hybrid assays. Yellow represents the fluorescent signals detected in BiFC assays. Gray indicates no detectable interaction signal. White indicates not analyzed. The percentage at the bottom of each subclade is the sequence identity of amino acids among the sequences from the four species. Pf, P. floridana; Ca, C. annuum; Sl, S. lycopersicum; St, S. tuberosum; At, Arabidopsis thaliana. B Southern-blotting analyses of MBP21 genes between Solanum and Physalis. Total DNA was isolated from S. tuberosum and P. floridana and digested with EcoR I and Hind III. C The expression of the indicated genes in Solanum and Physalis revealed by northern-blotting analysis. Total RNA was isolated from the leaves (Le), floral buds (FB), and flowers (Fl) of S. tuberosum and P. floridana. MADS-box gene-specific probes from S. tuberosum or P. floridana, as indicated, were used. D Microsynteny of the MBP21 subclade-containing region among the four indicated species from Solanum, Capsicum, and Physalis. The brown linear region represents the syntenic gene blocks of the MBP21 gene, and the gray linear region represents the syntenic regions of genes near the target gene. E StMBP21 expression in OE-StMBP21 P. floridana plants. Total RNA was isolated from the flowers of two independent transgenic lines and subjected to northern-blotting analyses. The indicated gene-specific probes were used. F Flower morphology. Compared to wild-type (WT) flowers, flowers from StMBP21-overexpressing transgenic plants (OE-StMBP21) were withered, implying that fertilization was inhibited. G Fruit morphology comparison. Wild type, WT; StMBP21-overexpressing transgenic Physalis plants, OE-StMBP21. Self-pollination (SP) occasionally occurred in the OE-StMBP21 flowers, and artificial pollination (AP) produced berries of similar size to the WT, indicating that female fertility was normally functional and that male function was reduced in OE-StMBP21 plants. Part of the Chinese lantern was removed to show the berry. Bar = 1.0 cm in (F, G)

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