Fig. 1
From: Two novel VCP missense variants identified in Japanese patients with multisystem proteinopathy
a–f: Histological analysis of the muscle biopsies from patients. a, d: Hematoxylin and eosin (HE), (b, e): modified Gomori trichrome (mGt), (c, f): ATPase (pH10.6). A group of atrophic fibers was observed in both patients (a, c, d, f). mGt staining revealed the presence of rimmed vacuoles (b, e, black arrows). Bar: 50 µm. g–l: Immunostaining for valosin-containing protein (VCP: g, i, j, l) and ubiquitin (h, i, k, l). VCP positive inclusions were co-stained with ubiquitin in both the nucleus (white arrowheads, DAPI positive) and the cytoplasm (white arrows, DAPI negative). Bar: 50 µm
