Fig. 1 | Hypertension Research

Fig. 1

From: ATP2B1 Gene Silencing Increases NO Production Under Basal Conditions Through the Ca2+/calmodulin/eNOS Signaling Pathway in Endothelial Cells

Fig. 1

ATP2B1 gene silencing results in increased basal NO production and eNOS activity in HUVECs. a Representative Western blotting results of PMCA1 protein and GAPDH. b The fold changes in PMCA1 protein relative to that in the control cells were quantified by densitometry (n = 4). Forty-eight hours after transfection, NO production (c) and eNOS activity (d) were detected in HUVECs. e Representative Western blotting results of p-eNOS, eNOS and GAPDH (n = 3). f Fold changes in phosphor-eNOS vs. total eNOS relative to its basal levels in control cells. *P < 0.05

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