Fig. 4

Effects of drug withdrawal on vascular function 21 days after implantation of an osmotic pump in DM mice. Cumulative concentration–response curves to ACh (a; 10−9–10−5 M), SNP (b; 10−10–10−5 M), clonidine (c; 10−9–10−6 M) and insulin (d; 10−9–3 × 10−6 M) in aortas from control, DM, clonidine-treated DM, GRK2-inhibitor-treated DM or clonidine/GRK2-inhibitor-cotreated DM mice. Values represent the mean ± SE; n = 5. *P < 0.05, **P < 0.01 vs. Control. ##P < 0.01 vs. DM mice