Fig. 3


APP and Aβ expression determined by immunostaining, immunoblotting, and ELISA in the cortices and hippocampi from each group. a, b Immunostaining and immunoblotting to determine APP expression levels in the cortex and hippocampus. c Immunostaining to show Aβ deposition in the cortex and hippocampus. d Quantification of the APP immunoblotting (n = 5). e Quantification of Aβ deposition by ELISA assay in the cortices and hippocampi from each group (n = 5). The values are shown as the mean ± SD, ***p < 0.001. L-NAME, Nω-nitro l-arginine methyl ester; PE, physical exercise; SHRSP, stroke-prone spontaneously hypertensive rats; WKY, Wistar Kyoto rats. Scale bar = 25 μm