Fig. 2
eNOS activation and NO production induced by SOCE in isolated aortic ECs. A Representative immunoblotting for eNOS phosphorylation at Ser1177 and total eNOS with or without SOCE by calcium add-back. B Quantification of densitometry analysis of Fig. 2A. C The representative traces for NO production in isolated ECs induced by calcium add-back; the AUC from 0 min (the start of thapsigargin) to 15 min (calcium add-back) was calculated as the first peak and the one from 15 min to 20 min as the second peak in Fig. 2D. D Quantification of the AUC of NO production. The first peak indicates NO production by calcium release from intracellular stores, and the second by calcium influx from the extracellular space. Flox:n = 8, ECKO:n = 9, Error bars represent S.E.M
