Fig. 3
From: Application of laser scanning cytometry in vascular smooth muscle remodeling
Cell cycle events. a Event mapping of PASM cells, no treatment. Distinct event populations can be identified and color coded within scattergrams or histograms and the color-coded events mapped to their specific coordinates within recorded scan field images. b Cell cycle events. Max pixel extensions of Go/G1 and G2 populations. Condensation of Go/G1 events is shown as cyan and of G2 events is in magenta. Nuclear condensation results in the cell cycle event populations with extended max pixel values in scattergram plots. Nuclear condensation can be indicative of either mitotic or apoptotic processes. Event mapping shows that condensed nuclei have a morphology consistent with apoptosis processes occurring within the population of cultured cells. c The horizontal/vertical division lines can be positioned manually, creating sectors of defined size (quadrants), d The normal growing population in Go/G1 was predominant (97% of the total population)
