Fig. 4
From: Application of laser scanning cytometry in vascular smooth muscle remodeling
Quantification of cell cycle nuclear events by LSC. a Gallery and field image of region of interest (ROI), i.e., R4 (G2 events). X, Y scattergram of hypoxia together with stretch representing regions of interest. b Scattergram and quadrant statistics. Hypoxia without stretch (The predominant proportion of normal cells is in Go/G1 phase 84% of total, and a small proportion is in G2 phase 15%. S phases: 1%. No apoptosis). c Normoxia together with stretch. d Normoxia without stretch. e Apoptosis and cell cycle analysis. Hypoxia together with stretch: (Go/G1 = 88%, G2 = 12%, S = 0%, apoptosis = 0%). Normoxia with stretch: (Go/G1 86%, G2 13%, M 1%). Normoxia without stretch: (Go/G1 93%, G2 7%). f Statistics. The cytometer software contains a statistic package allowing for the selection of various statistical parameters for data analysis. For the present analysis, the statistical parameter “count” was used for the assessment of the percentage of nuclear events in various stages of the cell cycle. N = 3 biological replicates
