Fig. 5

Phantom contour analysis of cytoplasmic F:G actin ratios in cultured primary PASM cells. a Identification of background and cell associated phantom populations (long red channel). The cell-associated phantom contour population is used for the quantification of F-and G-actin fluorescence. b To remove the nuclear G-actin fluorescence from the total G-actin fluorescence, cell-associated phantoms must be separated further into nuclear and cytoplasmic populations. This is achieved through a scattergram plot of blue versus long red phantom fluorescence, the numerical values of such separation are shown in C. c Fluorescence quantification can be expressed either as the summed integrated fluorescence of all phantoms or as the mean integrated phantom fluorescence