Fig. 6

Isolation of nuclear-associated phantoms. a A scattergram blot of blue (Hoechst) nuclear fluorescence versus long red (F-actin) cytoplasmic fluorescence reveals distinct phantom populations: Phantoms with low blue/low long red and low blue/high long red fluorescence (yellow box). Phantoms with low blue/high long red and high blue/high long red fluorescence (orange box). b Cell-associated phantoms previously identified in the phantom histogram, map to regions of low blue/high long red and high blue/high long red fluorescence within the phantom scattergram. c Phantoms with both high blue/high long red fluorescence (green box) are likely the cell-associated-nuclear phantoms. ROI region of inetrest