Fig. 9
From: Application of laser scanning cytometry in vascular smooth muscle remodeling
a Examination of SM markers in hypertensive PASM or (b) normal hASM lysates to confirm the basal expression. b Levels of SM markers α-actin, β-actin, desmin in a concentration gradient-dependent manner that ranges from 1 to 10 µg hASM cell lysate in relation to different conditions of hypoxia and flexing. b Concentration gradient shows the basal levels of ERK1/2, pERK1/2, AKT, Src, as they are expressed in serum fed and serum deprived hASM, and the lack of pAKT expression. β-actin is a reference protein. Cropped gels are presented for both panels. N normoxia, F flexing, H hypoxia
