Table 1 Primer sequences for qPCR
From: Effects of empagliflozin on nondiabetic salt-sensitive hypertension in uninephrectomized rats
Gene | Forward (F) and reverse (R) primer sequences | PCR product (bp) | GenBank Accession No. |
|---|---|---|---|
PHD2 | F 5′-CTGGGACGCCAAGGTGA-3′ R 5′-CAATGTCAGCAAACTGG-3′ | 71 | XM_017590462.1 |
HO-1 | F 5-TCTATCGTGCTCGCATGAAC-3′ R 5′-CAGCTCCTCAAACAGCTCAA-3′ | 109 | NM_012580.2 |
ET-1 | F 5′-TCTACTTCTGCCACCTGGACAT-3′ R 5′-GAAGGGCTTCCTAGTCCATACG-3′ | 76 | XM_017600453.1 |
eNOS | F 5′-CACACTGCTAGAGGTGCTGGAA-3′ R 5′-TGCTGAGCTGACAGAGTAGTA-3′ | 109 | XM_006235872.1 |
COX-2 | F 5′-ACCAACGCTGCCACAACT-3′ R 5′-GGTTGGAACAGCAAGGATTT-3′ | 128 | S67722.1 |
VEGF | F 5′-CTTCCTATTCCCCTCTTAAATCGTG-3′ R 5′-CTACCTCTTTCCTCTGCTGATTTCC-3′ | 102 | NM_001287114.1 |
NLRP3 | F 5′-CAGACCTCCAAGACCACGACTG-3′ R 5′-CATCCGCAGCCAATGAACAGAG-3′ | 128 | NM_001191642.1 |
CASP1 | F 5′-TGCCTGGTCTTGTGACTTGGAG-3′ R 5′-ATGTCCTGGGAAGAGGTAGAAACG-3′ | 134 | NM_012762.2 |
ASC | F 5′-TTATGGAAGAGTCTGGAGCTGTGG-3′ R 5′-AATGAGTGCTTGCCTGTGTTGG-3′ | 102 | NM_172322.1 |
IL-1β | F 5′-GAGGCTGACAGACCCCAAAAGAT-3′ R 5′-GCACGAGGCATTTTTGTTGTTCA-3′ | 339 | NM_031512 |
MCP-1 | F 5′-GGTCTCTGTCACGCTTCTG-3′ R 5′-TTCTCCAGCCGACTCATTG-3′ | 145 | NM_031530 |
RANTES | F 5′-CACCTGCCTCCCCATATG-3′ R 5′-TTCCTTCGAGTGACAAAGACG-3′ | 146 | NM_031116 |
NOX1 | F 5′-GGAGTTGCAGGAGTCCTCATTTT-3′ R 5′-TTCTGCCGGGAGCGATAA-3′ | 119 | NM_053683.1 |
gp91phox | F 5′- AAAGGAGTGCCCAGTACCAAAGT-3′ R 5′-TACAGGAACATGGGACCCACTAT-3′ | 79 | AF298656 |
p47phox | F 5′-ACGCTCACCGAGTACTTCAACA-3′ R 5′-TCATCGGGCCGCACTTT-3′ | 96 | AY029167 |
P67phox | F 5′-GCTTCGGAACATGGTGTCTAAGA-3′ R 5′-AGAGTCAGGCAGTAGTTTTTCACTTG-3′ | 220 | AB002664 |
NOX4 | F 5′-AGAATGAGGATCCCAGAAAGCTT-3′ R 5′-ATGAGGAACAATACCACCACCAT-3′ | 89 | NM_053524.1 |
CuZn-SOD | F 5′-TGTGTCCATTGAAGATCGTGTGA-3′ R 5′-TCTTGTTTCTCGTGGACCACC-3′ | 85 | NM_017050 |
Mn-SOD | F 5′-TTAACGCGCAGATCATGCA-3′ R 5′-CCTCGGTGACGTTCAGATTGT-3′ | 76 | NM_017051 |
GAPDH | F 5′-AGACAGCCGCATCTTCTTGT-3′ R 5′-CTTGCCGTGGGTAGAGTCAT-3′ | 200 | XM_017593963 |
