Fig. 3

SGLT1 and SGLT2 expression in the cortex of rats with IVC ligation. A The mRNA level of Slc5a1 (Sglt1) and Slc5a2 (Sglt2) was measured by reverse transcription real-time quantitative polymerase chain reaction in the cortex. The mRNA expression was normalized to Rplp2, Ppia, and Pgk1. B Representative immunoblotting and quantitative data of SLC5A2 (SGLT2) in the cortex. The protein expression was normalized to GAPDH. Data are presented as individual values and mean ± SEM; the relative value of the right contralateral non-congested kidney without tofogliflozin treatment was arbitrarily set to “1”; n = 12 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant (P > 0.05) by Steel-Dwass test. R, right kidney; L, left kidney; CMC, rats without tofogliflozin treatment; Tofo, rats with tofogliflozin treatment, A.U., arbitrary unit. C Representative light micrographs of immunohistochemical staining for SGLT2 in the cortex. Scale bar = 100 µm. The corresponding figures are provided in Supplementary Fig. S6. Representative confocal micrographs of double immunofluorescence staining for D SLC5A2 (SGLT2; green) and tubular injury marker HAVCR1 (KIM1; red) and E SLC5A2 (SGLT2; red) and proximal tubules marker LRP2 (megalin; green). The nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 20 µm