Fig. 3

Evaluation of nNOS expression in the nerve fibers of the dorsal nerve. a Control group. b Diabetic ED group. c Epalrestat-treated group. d Statistical chart of the nNOS mean density among groups. The arrows indicate the nerve fibers expressing nNOS. *P < 0.01 compared to the control group; #P < 0.05 compared to the diabetic group. DED diabetic group, Epal epalrestat-treated group. Scale bars = 10 μm