Fig. 1
From: Discovery of long-range inhibitory signaling to ensure single axon formation
Local application of neurotrophins to an axon terminal induced remote minor neurite retraction. a Local application of neurotrophins induced remote minor neurite retraction. PBS, NT-3, or BDNF was locally applied to the axon terminal of a stage 3 hippocampal neuron, and the images following this application are presented. Yellow and white arrowheads indicate the retracting and resting minor neurites, respectively. Scale bar, 50 μm. b–d Time course of changes in the lengths of the axon (blue) and minor neurites (red) from a single neuron. e, f Axonal outgrowth (PBS = 14, NT-3 = 15, BDNF = 16 neurons from three independent experiments) and minor neurite outgrowth (PBS = 45, NT-3 = 39, BDNF = 31 neurites from three independent experiments) were measured. Error bars represent SEM. *P < 0.05 and **P < 0.01
