Table 1 Interactions between GST-Syt II/Syt I and HCB variants measured by BLI
From: Engineered botulinum neurotoxin B with improved efficacy for targeting human receptors
Ligand | HCB | k on (M−1s−1) | k on error | k off (s−1) | k off error | K D (µM) |
|---|---|---|---|---|---|---|
GST-m-Syt II | WT | 1.47 × 104 | 5.89 × 102 | 1.98 × 10−3 | 5.18 × 10−5 | 0.13 |
GST-h-Syt II | WT | N/A | N/A | N/A | N/A | >20 |
E1191M | 5.21 × 103 | 2.11 × 102 | 3.51 × 10−2 | 4.62 × 10−4 | 6.7 | |
E1191M, S1199W | 1.65 × 104 | 3.87 × 102 | 1.81 × 10−2 | 1.79 × 10−4 | 1.10 | |
E1191M, S1199Y | 1.56 × 104 | 3.72 × 102 | 1.13 × 10−2 | 1.74 × 10−4 | 0.72 | |
E1191M, W1178Q | 2.23 × 104 | 1.33 × 103 | 3.52 × 10−2 | 8.25 × 10−4 | 1.58 | |
E1191C, S1199W | 1.32 × 104 | 4.70 × 102 | 1.19 × 10−2 | 8.90 × 10−4 | 0.90 | |
E1191C, S1199Y | 1.47 × 104 | 4.96 × 102 | 1.44 × 10−2 | 1.91 × 10−4 | 0.98 | |
E1191C, W1178Q | 6.89 × 103 | 5.18 × 102 | 2.96 × 10−2 | 3.23 × 10−4 | 4.30 | |
E1191Q, S1199W | 8.28 × 103 | 5.50 × 102 | 2.21 × 10−2 | 3.34 × 10−4 | 2.67 | |
E1191Q, S1199Y | 2.42 × 104 | 1.16 × 103 | 2.85 × 10−2 | 4.60 × 10−4 | 1.18 | |
E1191Q, W1178Q | N/A | N/A | N/A | N/A | >20 | |
E1191V, S1199W | 1.86 × 104 | 6.49 × 102 | 1.30 × 10−2 | 2.44 × 10−4 | 0.70 | |
E1191V, S1199Y | 2.31 × 104 | 9.18 × 102 | 1.35 × 10−2 | 2.68 × 10−4 | 0.59 | |
E1191V, W1178Q | 6.08 × 103 | 4.64 × 102 | 1.83 × 10−2 | 2.75 × 10−4 | 3.01 | |
E1191M, S1199W, W1178Q | 1.52 × 104 | 3.49 × 102 | 1.72 × 10−2 | 1.71 × 10−4 | 1.13 | |
GST-h-Syt I | WT | N/A | N/A | N/A | N/A | >20 |
E1191M, S1199Y | 4.82 × 103 | 2.96 × 102 | 1.40 × 10−2 | 4.55 × 10−4 | 2.90 | |
E1191V, S1199Y | 2.71 × 103 | 9.11 × 101 | 1.58 × 10−2 | 2.10 × 10−4 | 5.82 |
