Fig. 1
From: Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine
Production of PV3 VLPs in plants. a N. benthamiana leaf 6 days after infiltration expressing PV sVLPs suffering severe necrosis from the high levels of 3CD. b Infiltrated N. benthamiana leaf 6 days after infiltration expressing PV sVLPs using the downregulated 3CD. c Western blot analysis of sucrose cushioned sample showing the strongest signal for VP1 in the 70% sucrose interface (IF). d Nycodenz gradient demonstrating the separation of the PV3 SktSC8 VLPs (top band) from contamination. e Western blot of Nycodenz gradient confirming that the top gray band contains most VP1. The lanes with the pre-stained size markers and the positive control (C+) have been duplicated in c and e as all the samples were originally analyzed on the same gel (Supplementary Fig. 1)
