Fig. 3
From: Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine
Structure determination of PV3 sVLPs. a Cryo-EM image of PV3 SktSC8 VLPs in vitreous ice. Examples of some VLPs are highlighted with black arrows. Scale bar, 50 nm. b 3D reconstruction of PV3 SktSC8 sVLP at 3.6 Å resolution, viewed along the two-fold axis. The surface is colored by radius from the center of the VLP from blue through green to red from the highest to the lowest radius. c A central slice through the VLP to show the empty internal surface viewed along the two-fold axis and colored as in b. d Cryo-EM density map at 3.6 Å resolution of PV3 SktSC8 sVLP looking at the VP1 protein pocket. VP1 is shown as sticks with carbon atoms colored yellow, nitrogen atoms in blue and oxygen in red. e Cryo-EM density map at 4.1 Å resolution of the PV3 SktSC8 sVLP with GPP3 pocket factor compound bound to the VP1 protein pocket. VP1 is shown as sticks and colored as in d. The GPP3 molecule is colored in green. Cryo-EM density maps are contoured at 1.0σ
