Fig. 4
From: Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine
Stabilizing mutations in PV3 sVLPs. a Mutations in the PV3 SktSC8 sVLP compared to wt sequence. Panels show a snapshot of cryo-EM density for each of the mutations present in the PV3 SktSC8 sVLP. Wt residues are shown as sticks colored orange, with mutated residues observed in the 3.6 Å cryo-EM map as green. Cryo-EM density maps are contoured at 1.0σ. b Cartoon representation of the asymmetric unit for the PV3 SktSC8 sVLP. Individual subunits of the protomer are colored blue (VP1), green (VP2), red (VP3) and yellow (VP4). Mutations are shown as spheres and colored magenta. The position of the GPP3 molecule observed bound to the VP1 protein pocket of the 4.1 Å structure of PV3 SktSC8 sVLP is shown as orange spheres
