Fig. 6
From: Co-option of the cardiac transcription factor Nkx2.5 during development of the emu wing
Impact of Nkx2.5 mis-expression throughout chick wing. a, b In situ hybridization for Nkx2.5 demonstrating viral mis-expression (arrowhead) in a the right wing bud 2 days post infection and b 4 days post infection. c, d control virus infection demonstrating unaltered wing morphology in (c) unfixed and d alcian blue-stained preparation of an embryo harvested 10 days after infection (n = 6). e–h Nkx2.5-expressing virus infection, e unfixed, and f alcian blue-stained preparation (n = 19) of an embryo harvested 6 days after infection demonstrating reduced wing growth along the entire proximodistal axis of the right wing (arrowhead) in comparison to uninfected left hand side, g unfixed, and h alcian blue-stained preparation of an embryo harvested 10 days after infection demonstrating extreme reduction of wing growth and digit loss (arrowhead). Scale bar = 2 mm
