Fig. 8
From: Identification of a hybrid myocardial zone in the mammalian heart after birth
Clonal analysis of single Hey2+ cardiomyocyte from fetal to postnatal stage. a Schematic figure showing four alternative recombination results (nGFP, YFP, RFP, and mCFP) by crossing Hey2-CreER with R26-Confetti line. b Schematic figure showing experimental strategy. c, f Whole-mount fluorescence view of E15.5 and P7 Hey2-CreER;R26-Confetti hearts. d, g Sectional view of clones in E14.5/E15.5 and P7 Hey2-CreER;R26-Confetti heart sections. Arrowheads indicate single color colonies. Dotted lines in g indicate epicardium. e, h Quantification of the percentage of clone numbers in the inner, middle, and outer layer in each heart. n = 18 for E14.5/E15.5; n = 51 for P7. i Cartoon image showing sparse labeling of Hey2+ cells at embryonic stage and their expansion and contribution to the middle and outer myocardium layer of postnatal heart. Scale bars, 1 mm in c, f; 100 µm in d, g
